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Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids
 

Testing Epithelial Permeability in Fetal Tissue-Derived Enteroids

Article DOI: 10.3791/64108-v
June 16th, 2022

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Summary June 16th, 2022

This protocol details the establishment of enteroids, a three-dimensional intestinal model, from fetal intestinal tissue. Immunofluorescent imaging of epithelial biomarkers was used for model characterization. Apical exposure of lipopolysaccharides, a bacterial endotoxin, using microinjection technique induced epithelial permeability in a dose-dependent manner measured by the leakage of fluorescent dextran.

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