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Journal / Biochemistry / Recopilación de datos crio-EM de una sola…
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Biochemistry

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JoVE Journal Biochemistry
Recopilación de datos crio-EM de una sola partícula con inclinación de la etapa utilizando Leginon
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Recopilación de datos crio-EM de una sola partícula con inclinación de la etapa utilizando Leginon

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022
July 1st, 2022 •
1, 1, 2, 2,3, 1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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El presente protocolo describe un esquema generalizado y fácil de implementar para la recopilación de datos inclinados de una sola partícula en experimentos crio-EM. Este procedimiento es especialmente útil para obtener un mapa EM de alta calidad para muestras que sufren un sesgo de orientación preferencial debido a la adherencia a la interfaz aire-agua.

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