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JoVE Journal Biochemistry
Estudio de las funciones y actividades del cotransportador neuronal K-Cl KCC2 mediante Western Blotting
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Estudio de las funciones y actividades del cotransportador neuronal K-Cl KCC2 mediante Western Blotting

Article DOI: 10.3791/64179-v • 10:08 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories

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El presente protocolo destaca la aplicación de la técnica Western blotting para estudiar las funciones y actividades del cotransportador neuronal K-Cl KCC2. El protocolo describe la investigación de la fosforilación de KCC2 en los sitios reguladores de quinasa Thr906/1007 a través de Western blotting. Además, los métodos adicionales para confirmar la actividad de KCC2 se destacan brevemente en este texto.

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Bioquímica Número 190
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