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Journal / Biochemistry / Studie av funktioner och aktiviteter av neuronala …
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Biochemistry

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JoVE Journal Biochemistry
Studie av funktioner och aktiviteter av neuronala K-Cl Co-Transporter KCC2 med hjälp av Western Blotting
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Studie av funktioner och aktiviteter av neuronala K-Cl Co-Transporter KCC2 med hjälp av Western Blotting

Article DOI: 10.3791/64179-v • 10:08 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories

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Detta protokoll belyser tillämpningen av western blotting-teknik för att studera funktioner och aktiviteter hos neuronal K-Cl-co-transporter KCC2. Protokollet beskriver undersökningen av KCC2-fosforylering vid kinasregleringsställen Thr906/1007 via western blotting. Dessutom markeras ytterligare metoder för att bekräfta KCC2-aktivitet kort i den här texten.

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Biokemi utgåva 190
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