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Journal / Immunology and Infection / Bestämning av tarmpermeabilitet med Lucifer…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bestämning av tarmpermeabilitet med Lucifer Yellow i en apikal-out enteroidmodell
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Bestämning av tarmpermeabilitet med Lucifer Yellow i en apikal-out enteroidmodell

Article DOI: 10.3791/64215-v • 09:48 min • July 27th, 2022
July 27th, 2022 •
1, 2, 2, 1, 1, 3, 1
1Division of Pediatric Surgery, Oklahoma Children's Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery, University of Oklahoma Health Sciences Center

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Det nuvarande protokollet beskriver en metod som använder lucifergul i en apikal-out enteroidmodell för att bestämma tarmpermeabilitet. Denna metod kan användas för att bestämma paracellulär permeabilitet i enteroider som modellerar inflammatoriska tarmsjukdomar såsom nekrotiserande enterokolit.

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Immunologi och infektion utgåva 185
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