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在根尖肠模型中使用路西法黄测定肠通透性

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Immunology and Infection

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JoVE Journal Immunology and Infection

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

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在根尖肠模型中使用路西法黄测定肠通透性

DOI:

10.3791/64215-v

•

09:48 min

•

July 27, 2022

•

Heather Liebe, Camille Schlegel, Xue Cai, Alena Golubkova, Tyler Leiva, William L. Berry, Catherine J. Hunter

¹Division of Pediatric Surgery,Oklahoma Children’s Hospital, ²Oklahoma University Health Sciences Center, ³Department of Surgery,University of Oklahoma Health Sciences Center

Chapters

00:04Introduction
00:59Generation of Apical‐Out (AO) Enteroids
03:08Induction of Experimental Necrotizing Enterocolitis (NEC)
05:06Measurement of Paracellular Permeability Utilizing Lucifer Yellow (LY)
07:49Results: Determining the Intestinal Permeability Using Lucifer Yellow in an Apical‐Out Enteroid Model
09:07Conclusion

Summary

Automatic Translation

本协议概述了一种在顶端肠模型中利用荧光花黄来确定肠道通透性的方法。该方法可用于确定模拟炎症性肠病（如坏死性小肠结肠炎）的肠样肠的细胞旁通透性。

Tags

Keywords: Lucifer Yellow Apical-out Enteroid Model Intestinal Permeability Paracellular Pathways Necrotizing Enterocolitis Cell Recovery Solution Centrifugation 3D Model 24-well Plate AO Enteroids

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