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Bestemmelse af tarmpermeabilitet ved hjælp af Lucifer Yellow i en apikal enteroidmodel
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
DOI:

09:48 min

July 27, 2022

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Chapters

  • 00:04Introduction
  • 00:59Generation of Apical‐Out (AO) Enteroids
  • 03:08Induction of Experimental Necrotizing Enterocolitis (NEC)
  • 05:06Measurement of Paracellular Permeability Utilizing Lucifer Yellow (LY)
  • 07:49Results: Determining the Intestinal Permeability Using Lucifer Yellow in an Apical‐Out Enteroid Model
  • 09:07Conclusion

Summary

Automatic Translation

Den nuværende protokol skitserer en metode, der anvender lucifer gul i en apikal-out enteroid model til bestemmelse af tarmpermeabilitet. Denne metode kan bruges til at bestemme paracellulær permeabilitet hos enteroider, der modellerer inflammatoriske tarmsygdomme såsom nekrotiserende enterocolitis.

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