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Journal / Immunology and Infection / Bestemmelse af tarmpermeabilitet ved hjælp af…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bestemmelse af tarmpermeabilitet ved hjælp af Lucifer Yellow i en apikal enteroidmodel
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Bestemmelse af tarmpermeabilitet ved hjælp af Lucifer Yellow i en apikal enteroidmodel

Article DOI: 10.3791/64215-v • 09:48 min • July 27th, 2022
July 27th, 2022 •
1, 2, 2, 1, 1, 3, 1
1Division of Pediatric Surgery, Oklahoma Children's Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery, University of Oklahoma Health Sciences Center

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Den nuværende protokol skitserer en metode, der anvender lucifer gul i en apikal-out enteroid model til bestemmelse af tarmpermeabilitet. Denne metode kan bruges til at bestemme paracellulær permeabilitet hos enteroider, der modellerer inflammatoriske tarmsygdomme såsom nekrotiserende enterocolitis.

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Immunologi og infektion udgave 185
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