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Immunology and Infection

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JoVE Journal Immunology and Infection
Intestinale permeabiliteit bepalen met lucifergeel in een apical-out enteroïde model
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Intestinale permeabiliteit bepalen met lucifergeel in een apical-out enteroïde model

Article DOI: 10.3791/64215-v • 09:48 min • July 27th, 2022
July 27th, 2022 •
1, 2, 2, 1, 1, 3, 1
1Division of Pediatric Surgery, Oklahoma Children's Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery, University of Oklahoma Health Sciences Center

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Het huidige protocol schetst een methode die lucifergeel gebruikt in een apicale enteroïde model om de darmdoorlaatbaarheid te bepalen. Deze methode kan worden gebruikt om paracellulaire permeabiliteit te bepalen bij enteroïden die inflammatoire darmziekten modelleren, zoals necrotiserende enterocolitis.

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Immunologie en infectie nummer 185
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