Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Zellfreie Proteinsynthese aus…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Zellfreie Proteinsynthese aus Exonuklease-defizienten Zellextrakten unter Verwendung linearer DNA-Vorlagen
Dissection, MicroCT Scanning and Morphometric Analyses of the Baculum
Journal (Bioengineering)
Dissection, MicroCT Scanning and Morphometric Analyses of the Baculum
Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Journal (Bioengineering)
Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
 
Click here for the English version

Zellfreie Proteinsynthese aus Exonuklease-defizienten Zellextrakten unter Verwendung linearer DNA-Vorlagen

Article DOI: 10.3791/64236-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1, 2,3, 4, 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier wird ein Protokoll zur Herstellung und Pufferkalibrierung von Zellextrakten aus Exonuklease-V-Knockout-Stämmen von Escherichia coli BL21 Rosetta2 (ΔrecBCD und ΔrecB) vorgestellt. Dies ist ein schneller, einfacher und direkter Ansatz für die Expression in zellfreien Proteinsynthesesystemen mit linearen DNA-Vorlagen.

Tags

Bioengineering Ausgabe 186
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter