Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Lineer DNA Şablonlarını Kullanarak Ekzonükleaz…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Lineer DNA Şablonlarını Kullanarak Ekzonükleaz Eksikliği Olan Hücresel Ekstraktlardan Hücresiz Protein Sentezi
Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
Journal (Bioengineering)
Simultaneous Quantification of Selected Kynurenines Analyzed by Liquid Chromatography-Mass Spectrometry in Medium Collected from Cancer Cell Cultures
Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
Journal (Bioengineering)
Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
 
Click here for the English version

Lineer DNA Şablonlarını Kullanarak Ekzonükleaz Eksikliği Olan Hücresel Ekstraktlardan Hücresiz Protein Sentezi

Article DOI: 10.3791/64236-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1, 2,3, 4, 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Burada sunulan, Escherichia coli BL21 Rosetta2'nin (ΔrecBCD ve ΔrecB) ekzonükleaz V nakavt suşlarından hücre ekstraktlarının hazırlanması ve tampon kalibrasyonu için bir protokoldür. Bu, lineer DNA şablonlarını kullanarak hücresiz protein sentez sistemlerinde ekspresyon için hızlı, kolay ve doğrudan bir yaklaşımdır.

Tags

Biyomühendislik Sayı 186
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter