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Journal / Bioengineering / Celvrije eiwitsynthese uit exonuclease-deficiënte …
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Bioengineering

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JoVE Journal Bioengineering
Celvrije eiwitsynthese uit exonuclease-deficiënte cellulaire extracten met behulp van lineaire DNA-sjablonen
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Celvrije eiwitsynthese uit exonuclease-deficiënte cellulaire extracten met behulp van lineaire DNA-sjablonen

Article DOI: 10.3791/64236-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1, 2,3, 4, 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
* These authors contributed equally

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Hier wordt een protocol gepresenteerd voor de bereiding en bufferkalibratie van celextracten uit exonuclease V knock-out stammen van Escherichia coli BL21 Rosetta2 (ΔrecBCD en ΔrecB). Dit is een snelle, eenvoudige en directe benadering voor expressie in celvrije eiwitsynthesesystemen met behulp van lineaire DNA-sjablonen.

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Bio-engineering Nummer 186
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