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JoVE Journal Bioengineering
Cellefri proteinsyntese fra eksonuklease-mangelfulle cellulære ekstrakter ved bruk av lineære DNA-maler
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Cellefri proteinsyntese fra eksonuklease-mangelfulle cellulære ekstrakter ved bruk av lineære DNA-maler

Article DOI: 10.3791/64236-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1, 2,3, 4, 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
* These authors contributed equally

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Presentert her er en protokoll for fremstilling og bufferkalibrering av celleekstrakter fra eksonuklease V knockout-stammer av Escherichia coli BL21 Rosetta2 (ΔrecBCD og ΔrecB). Dette er en rask, enkel og direkte tilnærming for ekspresjon i cellefrie proteinsyntesesystemer ved hjelp av lineære DNA-maler.

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Bioteknologi utgave 186
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