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Journal / Bioengineering / 선형 DNA 주형을 활용한 엑소뉴클레아제 결핍 세포 추출물의 무세포 단백질…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
선형 DNA 주형을 활용한 엑소뉴클레아제 결핍 세포 추출물의 무세포 단백질 합성
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Isokinetic Robotic Device to Improve Test-Retest and Inter-Rater Reliability for Stretch Reflex Measurements in Stroke Patients with Spasticity
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Isokinetic Robotic Device to Improve Test-Retest and Inter-Rater Reliability for Stretch Reflex Measurements in Stroke Patients with Spasticity
 
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선형 DNA 주형을 활용한 엑소뉴클레아제 결핍 세포 추출물의 무세포 단백질 합성

Article DOI: 10.3791/64236-v • 08:11 min • August 9th, 2022
August 9th, 2022 •
*1, *1, 1, 2,3, 4, 1
1INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, 2Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), 3Medical Faculty, University of Würzburg, 4Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier
* These authors contributed equally

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여기에 제시된 것은 대장 균 BL21 Rosetta2 (ΔrecBCD 및 ΔrecB)의 엑소 뉴 클레아 제 V 녹아웃 균주로부터 세포 추출물의 제조 및 완충액 교정을위한 프로토콜입니다. 이것은 선형 DNA 주형을 사용하는 무세포 단백질 합성 시스템에서 발현을 위한 빠르고 쉽고 직접적인 접근 방식입니다.

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생명 공학 186 호
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