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一种微创、准确、高效的小鼠胸腺内注射技术
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Minimally Invasive, Accurate, and Efficient Technique for Intrathymic Injection in Mice
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一种微创、准确、高效的小鼠胸腺内注射技术

DOI:
10.3791/64309-v

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07:17 min

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August 23, 2022

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, , ,
1Department of Radiology,Hackensack University Medical Center, 2Center for Discovery and Innovation,Hackensack Meridian Health, 3Department of Pediatrics,Hackensack University Medical Center, 4Department of Oncology,Georgetown University

Chapters

  • 00:04Introduction
  • 00:59Animal Preparation
  • 01:47Preparation of the Ultrasound Machine and Sterile Field
  • 03:14Imaging and Locating the Thymus
  • 03:55Injection of the Thymus
  • 05:45Results: An Ultrasound‐Guided Free‐Hand Intrathymic Injection in Mice
  • 06:47Conclusion

Summary

Automatic Translation

Automatic Translation

本协议描述了为小鼠胸腺内注射建立的介入放射学程序，以避免开放手术的风险并提高盲经皮注射的准确性。

Tags

Minimally InvasiveAccurateEfficientIntrathymic InjectionMiceNon-surgicalUltrasound-guidedThymusAtrophyImmunosuppressionAnesthesiaDisinfectionUltrasound SettingsSterile Technique

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