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大肠杆菌
抗生素持久性的群体和单细胞分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Population and Single-Cell Analysis of Antibiotic Persistence in
Escherichia coli
Please note that all translations are automatically generated.
Click here for the English version.
大肠杆菌
抗生素持久性的群体和单细胞分析
DOI:
10.3791/64550-v
•
12:29 min
•
March 24, 2023
•
Thierry Oms*
1
,
Tatjana Schlechtweg*
1
,
Julien Cayron
,
Laurence Van Melderen
1
Bacterial Genetics and Physiology, Département de Biologie Moléculaire, Faculté des Sciences
,
Université Libre de Bruxelles (ULB)
Chapters
00:05
Introduction
00:31
Cell Culture and Growth Curve
02:21
Determination of Minimal Inhibitory Concentration of Antibiotics
04:03
Time‐Kill Assay
06:24
Microfluidic Time‐Lapse Microscopy Imaging
09:57
Results: Population and Single‐Cell Analysis of Antibiotic Persistence
11:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
抗生素持久性描述了敏感等基因人群中的小亚群暂时耐受高剂量杀菌抗生素的能力。本协议结合了在将
大肠杆菌
暴露于致死剂量的氧氟沙星后在分子和细胞水平上表征抗生素持久性表型的方法。
Tags
Antibiotic Persistence
Escherichia Coli
Single-cell Analysis
Population Analysis
Ofloxacin
Persister Cells
Microbiology Assays
Live Imaging
Molecular Characterization
Cellular Characterization
MOPS Growth Medium
LB Agar
Microplate Reader
Antibiotic Gradient
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