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Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning
DOI:

08:57 min

February 24, 2023

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Chapters

  • 00:04Introduction
  • 00:43Dissection, Trituration, and Immunopanning
  • 07:54Results: DRG Culture Neuronal Enrichment by Immunopanning
  • 08:31Conclusion

Summary

Automatic Translation

This paper describes an immunopanning protocol for adult mouse dorsal root ganglia. By adhering antibodies to culture plates, we can negatively select and remove non-neuronal cells. We show that the cultures are enriched for neurons using this protocol, allowing for an in-depth study of neuronal responses to manipulation.

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