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通过免疫淘选富集成年小鼠背根神经节神经元培养物
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Neuroscience
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JoVE Journal Neuroscience
Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning
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通过免疫淘选富集成年小鼠背根神经节神经元培养物

DOI:
10.3791/64603-v

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08:57 min

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February 24, 2023

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, ,
1Neuroscience and Mental Health Institute,University of Alberta, 2Department of Medicine, Division of Neurology,University of Alberta, 3Department of Medical Microbiology and Immunology,University of Alberta, 4Department of Pharmacology,University of Alberta, 5Department of Anesthesiology and Pain Medicine,University of Alberta

Chapters

  • 00:04Introduction
  • 00:43Dissection, Trituration, and Immunopanning
  • 07:54Results: DRG Culture Neuronal Enrichment by Immunopanning
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

本文描述了一种成年小鼠背根神经节的免疫平移方案。通过将抗体粘附到培养板上，我们可以负性地选择和去除非神经元细胞。我们表明，使用该协议为神经元富集培养物，从而可以深入研究神经元对操作的反应。

Tags

Keywords: DRG CultureImmunopanningNeuron EnrichmentDorsal Root GangliaPrimary CultureNeuronal SelectionNon-neuronal Cell Depletion

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