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Journal / Immunology and Infection / Detección de células T polifuncionales en niños…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Detección de células T polifuncionales en niños vacunados con la vacuna contra la encefalitis japonesa mediante la técnica de citometría de flujo
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Detección de células T polifuncionales en niños vacunados con la vacuna contra la encefalitis japonesa mediante la técnica de citometría de flujo

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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El presente protocolo combina la estimulación ex vivo y la citometría de flujo para analizar los perfiles de células T polifuncionales (T PF) en células mononucleares de sangre periférica (PBMC) en niños vacunados contra el virus de la encefalitis japonesa (JEV). El método de detección y el esquema de color de citometría de flujo de los TPF específicos de JEV se probaron para proporcionar una referencia para estudios similares.

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Inmunología e infección Número 187 Células T polifuncionales Encefalitis japonesa vacunación citometría de flujo
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