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Journal / Immunology and Infection / Japon Ensefalit Aşısı ile Aşılanan Çocuklarda Çok …
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Japon Ensefalit Aşısı ile Aşılanan Çocuklarda Çok Fonksiyonlu T Hücrelerinin Akım Sitometri Tekniği ile Saptanması
Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization
Journal (Immunology and Infection)
Evaluation of Blood Lactate and Plasma Insulin During High-intensity Exercise by Antecubital Vein Catheterization
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
Journal (Immunology and Infection)
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
 
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Japon Ensefalit Aşısı ile Aşılanan Çocuklarda Çok Fonksiyonlu T Hücrelerinin Akım Sitometri Tekniği ile Saptanması

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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Mevcut protokol, Japon ensefalit virüsü (JEV) aşılı çocuklarda periferik kan mononükleer hücrelerinde (PBMC'ler) polifonksiyonel T hücresi (TPF) profillerini analiz etmek için ex vivo stimülasyon ve akış sitometrisini birleştirmektedir. JEV'e özgü TPF'lerin tespit yöntemi ve akış sitometrisi renk şeması, benzer çalışmalar için bir referans sağlamak üzere test edilmiştir.

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İmmünoloji ve Enfeksiyon Sayı 187 Polifonksiyonel T hücreleri Japon ensefaliti aşılama akım sitometrisi
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