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Journal / Immunology and Infection / フローサイトメトリー法 による…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
フローサイトメトリー法 による 日本脳炎ワクチン接種小児における多機能性T細胞の検出
Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
Journal (Immunology and Infection)
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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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フローサイトメトリー法 による 日本脳炎ワクチン接種小児における多機能性T細胞の検出

Article DOI: 10.3791/64671-v • 09:37 min • September 23rd, 2022
September 23rd, 2022 •
*1,2, *1,2,3, 1,2, 1,2, 1,2, 4, 1,2, 1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
* These authors contributed equally

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Summary
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本プロトコルは、 ex vivo 刺激とフローサイトメトリーを組み合わせて、日本脳炎ウイルス(JEV)ワクチン接種小児内の末梢血単核球(PBMC)の多機能T細胞(TFP)プロファイルを分析します。JEV特異的TPFの検出方法とフローサイトメトリーの配色を試験し、同様の研究の参考資料を提供しました。

Tags

免疫感染症学、第187号、多機能T細胞、日本脳炎、ワクチン接種、フローサイトメトリー
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