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Journal / Biology / استجواب مزدوج خاص بالخلية ل Epigenome المبيض…
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استجواب مزدوج خاص بالخلية ل Epigenome المبيض للفأر و Transcriptome
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Journal (Biology)
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Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
Journal (Biology)
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
 
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استجواب مزدوج خاص بالخلية ل Epigenome المبيض للفأر و Transcriptome

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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في هذا البروتوكول ، تم تحسين طريقة تنقية تقارب الريبوسوم (TRAP) وعزل النوى الموسومة في أنواع معينة من الخلايا (INTACT) للاستجواب المزدوج لنسخة المبيض الخاصة بالخلية و epigenome باستخدام نموذج الماوس NuTRAP الذي تم عبوره إلى خط الماوس Cyp17a1-Cre.

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هذا الشهر في JoVE ، العدد 192 ،
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