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Journal / Biology / 小鼠卵巢表观基因组和转录组的细胞特异性配对询问…
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小鼠卵巢表观基因组和转录组的细胞特异性配对询问
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小鼠卵巢表观基因组和转录组的细胞特异性配对询问

Article DOI: 10.3791/64765-v • 12:25 min • February 24th, 2023
February 24th, 2023 •
*1,2,3, *1,3, 1, 2, 2, 4, 2,3, 1,3
1Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, 2Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, 3Oklahoma City Veterans Affairs Medical Center, 4Nutrition College, Federal University of Pelotas
* These authors contributed equally

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在该协议中，翻译核糖体亲和纯化（TRAP）方法和特定细胞类型（INTACT）方法中标记的细胞核的分离针对使用NuTRAP小鼠模型交叉到Cyp17a1-Cre小鼠系的细胞特异性卵巢转录组和表观基因组的配对询问进行了优化。

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