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Biochemistry

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JoVE Journal Biochemistry
Onderzoekende RNA-structuur met dimethylsulfaatmutatieprofilering met sequencing in vitro en in cellen
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
Journal (Biochemistry)
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
Journal (Biochemistry)
An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
 
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Onderzoekende RNA-structuur met dimethylsulfaatmutatieprofilering met sequencing in vitro en in cellen

Article DOI: 10.3791/64820-v • 10:34 min • December 9th, 2022
December 9th, 2022 •
1, 1
1Department of Microbiology, Harvard Medical School

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Het protocol geeft instructies voor het modificeren van RNA met dimethylsulfaat voor mutatieprofileringsexperimenten. Het omvat in vitro en in vivo tasten met twee alternatieve bibliotheekvoorbereidingsmethoden.

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Biochemie Nummer 190
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