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Journal / Biochemistry / Sondering av RNA-struktur med…
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Biochemistry

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JoVE Journal Biochemistry
Sondering av RNA-struktur med dimetylsulfatmutasjonsprofilering med sekvensering in vitro og i celler
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Sondering av RNA-struktur med dimetylsulfatmutasjonsprofilering med sekvensering in vitro og i celler

Article DOI: 10.3791/64820-v • 10:34 min • December 9th, 2022
December 9th, 2022 •
1, 1
1Department of Microbiology, Harvard Medical School

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Protokollen gir instruksjon for modifisering av RNA med dimetylsulfat for mutasjonsprofileringseksperimenter. Det inkluderer in vitro og in vivo sondering med to alternative biblioteksforberedelsesmetoder.

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Biokjemi utgave 190
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