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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

JoVE Journal
Medicine

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JoVE Journal Medicine

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

DOI:

10.3791/64875-v

•

05:51 min

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March 17, 2023

•

Yang Li*^1,2, Xue Qiao*¹, Xuechuan Hong²

¹College of Science, Research Center for Ecology, Laboratory of Extreme Environmental Biological Resources and Adaptive Evolution,Tibet University, ²State Key Laboratory of Virology, Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (MOE) and Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals,Wuhan University School of Pharmaceutical Sciences

Chapters

00:04Introduction
00:52Preparation for NIR‐II Imaging
01:36Preparation of Water‐Suspensible Nanoprobe
02:48In Vivo NIR‐II Fluorescence Imaging
03:41Results: Vascular and Tumor Imaging Using HLY1 Dots as NIR‐II Fluorescence Probe
05:22Conclusion

Summary

Automatic Translation

The present protocol describes a detailed, real-time NIR-II fluorescence imaging operation of a mouse using a NIR-II optics imaging device.

Tags

Keywords: NIR-II Fluorescence Probe Vascular Imaging Tumor Imaging NIR-II Window HLY1 Dye DSPE-PEG-2K Nanoprecipitation Aggregation-induced Emission (AIE)In Vivo Imaging Optical NIR-II Imaging System

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