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Mapeamento Óptico de Coração de Camundongos RyR2R2474S Knock-In de Taquicardia Ventricular Polimórfica Catecolaminérgica
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Mapeamento Óptico de Coração de Camundongos RyR2R2474S Knock-In de Taquicardia Ventricular Polimórfica Catecolaminérgica

Article DOI: 10.3791/65082-v • 09:36 min • December 22nd, 2023
December 22nd, 2023 •
*1,2, *1, 1, 1, 1, 1, 3, 1, 1,2, 1,4, 1
1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, 2Department of Cardiology, the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 4Department of Pharmacology, University of Oxford
* These authors contributed equally

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Este protocolo introduz o mapeamento óptico dual-dye de corações de camundongos obtidos de animais selvagens e knock-in afetados por taquicardia ventricular polimórfica catecolaminérgica, incluindo medidas eletrofisiológicas de voltagem transmembrana e transientes intracelulares de Ca2+ com alta resolução temporal e espacial.

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Este mês no JoVE edição 202
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