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Dual-Dye optisk kartlegging av hjerter fra RyR2R2474S Knock-In mus av katekolaminerg polymorf ventrikulær takykardi
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Dual-Dye optisk kartlegging av hjerter fra RyR2R2474S Knock-In mus av katekolaminerg polymorf ventrikulær takykardi

Article DOI: 10.3791/65082-v • 09:36 min • December 22nd, 2023
December 22nd, 2023 •
*1,2, *1, 1, 1, 1, 1, 3, 1, 1,2, 1,4, 1
1Key Laboratory of Medical Electrophysiology of Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease, Institute of Cardiovascular Research, Southwest Medical University, 2Department of Cardiology, the Affiliated Hospital of Southwest Medical University, 3Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 4Department of Pharmacology, University of Oxford
* These authors contributed equally

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Denne protokollen introduserer dual-dye optisk kartlegging av musehjerter oppnådd fra villtype og knock-in dyr påvirket av katekolaminerg polymorf ventrikulær takykardi, inkludert elektrofysiologiske målinger av transmembranspenning og intracellulære Ca2+ transienter med høy temporal og romlig oppløsning.

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