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Journal / Developmental Biology / Effiziente Vaskularisierung von Nierenorganoiden…
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JoVE Journal Developmental Biology
Effiziente Vaskularisierung von Nierenorganoiden durch intracelome Transplantation in Hühnerembryonen
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Effiziente Vaskularisierung von Nierenorganoiden durch intracelome Transplantation in Hühnerembryonen

Article DOI: 10.3791/65090-v • 07:35 min • February 17th, 2023
February 17th, 2023 •
1,2, 1,2, 3, 1,2,4, 1,2,4
1Department of Internal Medicine - Nephrology, Leiden University Medical Center, 2Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, 3IBPS, CNRS UMR7622, Developmental Biology Laboratory, Sorbonne Université, 4The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center

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In dieser Arbeit stellen wir ein detailliertes Protokoll für die Transplantation von Nierenorganoiden in die Zölomhöhle von Hühnerembryonen vor. Diese Methode induziert eine Vaskularisierung und eine verbesserte Reifung der Organoide innerhalb von 8 Tagen und kann verwendet werden, um diese Prozesse auf effiziente Weise zu untersuchen.

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