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Journal / Developmental Biology / Vascolarizzazione efficiente degli organoidi…
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JoVE Journal Developmental Biology
Vascolarizzazione efficiente degli organoidi renali attraverso il trapianto di intracelomica negli embrioni di pollo
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Vascolarizzazione efficiente degli organoidi renali attraverso il trapianto di intracelomica negli embrioni di pollo

Article DOI: 10.3791/65090-v • 07:35 min • February 17th, 2023
February 17th, 2023 •
1,2, 1,2, 3, 1,2,4, 1,2,4
1Department of Internal Medicine - Nephrology, Leiden University Medical Center, 2Einthoven Laboratory of Vascular and Regenerative Medicine, Leiden University Medical Center, 3IBPS, CNRS UMR7622, Developmental Biology Laboratory, Sorbonne Université, 4The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center

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Qui, presentiamo un protocollo dettagliato per il trapianto di organoidi renali nella cavità celomica degli embrioni di pollo. Questo metodo induce la vascolarizzazione e una maggiore maturazione degli organoidi entro 8 giorni e può essere utilizzato per studiare questi processi in modo efficiente.

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