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Journal
/
Biology
/
测量体
外
分化的离
体
小鼠脂肪组织和原代前脂肪细胞的脂肪分解率
JoVE Journal
Biology
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JoVE Journal
Biology
Measuring the Rate of Lipolysis in
Ex Vivo
Murine Adipose Tissue and Primary Preadipocytes Differentiated
In Vitro
Please note that all translations are automatically generated.
Click here for the English version.
测量体
外
分化的离
体
小鼠脂肪组织和原代前脂肪细胞的脂肪分解率
DOI:
10.3791/65106-v
•
09:41 min
•
March 17, 2023
•
Pania E. Bridge-Comer
,
Shannon M. Reilly
1
Weill Center for Metabolic Health, Department of Medicine
,
Weill Cornell Medicine
Chapters
00:04
Introduction
01:00
Preparation of Buffers and Collection Plates
01:36
Preparation of Primary Preadipocyte Samples
02:15
Preparation of Ex Vivo Culture Samples
03:44
Lipolysis Assay
04:33
Free Fatty Acid (FFA) Colorimetric Assay
05:46
Glycerol Colorimetric Assay
06:34
Calculation of Lipolytic Rate
07:18
Results: Basal and Stimulated Lipolytic Rates of In Vitro Differentiated Preadipocytes and Ex Vivo Adipose Tissue
09:09
Conclusion
Summary
Automatic Translation
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Automatic Translation
脂肪细胞中的甘油三酯脂肪分解是导致游离脂肪酸和甘油释放的重要代谢过程。在这里,我们提供了一个详细的方案来测量小鼠脂肪细胞和
离体
脂肪组织中的基础和刺激脂肪分解。
Tags
Keywords: Lipolysis
Preadipocytes
Murine
Ex Vivo
In Vitro
Free Fatty Acids
BSA
DMEM
Insulin
Differentiation
DPBS
Stimulation
Control
Serial Sampling
Measurement
Optimization
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