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Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut
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Chapters
- 00:05Title
- 01:47Microinjection of Nymphal Ixodes scapularis Ticks
- 04:50B. burgdorferi Localization by Confocal Immunofluorescence Microscopy
- 08:23Representative Tick Injection Results
- 08:58Conclusion
Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.
