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1Department of Chemical and Biological Engineering,Illinois Institute of Technology, 2Department of Biomedical Engineering,Illinois Institute of Technology, 3Department of Biomedical Engineering,University of California at Irvine, 4Wake Forest Institute for Regenerative Medicine and Department of Biomedical Engineering,Wake Forest University Health Sciences, 5Research Service,Hines Veterans Administration Hospital
Chapters
- 00:05Title
- 01:37Alginate Microbead Preparation
- 04:46Coating the Microbeads with Poly-L-ornithine
- 07:59Creating the Outer Alginate Layer
- 09:22Representative Results
In the following sections, we outline procedures for the preparation of alginate microspheres for use in biomedical applications. We specifically illustrate a technique for creating multilayered alginate microspheres for the dual purpose of cell and protein encapsulation as a potential treatment for type 1 diabetes.
Tags
Alginate MicrospheresBiomedical ApplicationsHydrophilic NatureBiocompatibilityPhysical ArchitectureCell EncapsulationDrug DeliveryStem Cell CultureIslets EncapsulationType I Diabetes TreatmentMicrovascular Network FormationOxygen TransportGlucose TransportVital Nutrients TransportFibroblast Growth Factor-1 (FGF-1)Sustained ReleaseGrowth Factor DeliveryBlood Vessel FormationFunctional Graft Outcomes
