Journal
/
Medicine
/
Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

Adaptation of Semiautomated Circulating Tumor Cell (CTC) Assays for Clinical and Preclinical Research Applications

DOI:
10.3791/51248-v

•

14:14 min

•

February 28, 2014

•

, , ,
1London Regional Cancer Program,London Health Sciences Centre, 2Department of Anatomy & Cell Biology, Schulich School of Medicine and Dentistry,Western University, 3Special Hematology/Flow Cytometry,London Health Sciences Centre, 4Lawson Health Research Institute, 5Department of Oncology,Western University

Chapters

  • 00:05Title
  • 01:42Human Blood Sample Preparation and Processing
  • 03:25Sample Scanning on the Analysis Instrument
  • 04:58CTC Characterization for User-defined Markers Using the CSS
  • 06:08Adaptation of the Standard CSS Protocol: Preclinical Mouse Models
  • 07:42CTC Staining
  • 09:20Scanning of Manually Separated Samples
  • 10:13Results: CTC Assays in Clinical and Preclinical Research
  • 13:43Conclusion

Summary

Automatic Translation

Automatic Translation

Circulating tumor cells (CTCs) are prognostic in several metastatic cancers. This manuscript describes the gold standard CellSearch system (CSS) CTC enumeration platform and highlights common misclassification errors. In addition, two adapted protocols are described for user-defined marker characterization of CTCs and CTC enumeration in preclinical mouse models of metastasis using this technology.

Tags

Keywords: Semiautomated CTC AssayCirculating Tumor CellsCellSearch SystemCTC DetectionCTC EnumerationCTC CharacterizationCTC CapturePreclinical Mouse ModelsMetastasisCTC Misclassification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51248?article_type=v

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
check_url/51248?article_type=v

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
check_url/51248?article_type=v

A Novel High-resolution In vivo Imaging Technique to Study the Dynamic Response of Intracranial Structures to Tumor Growth and Therapeutics
check_url/51248?article_type=v

In vivo 19F MRI for Cell Tracking
check_url/51248?article_type=v

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
check_url/51248?article_type=v

Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
check_url/51248?article_type=v

Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics
check_url/51248?article_type=v

Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model
check_url/51248?article_type=v

Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells
check_url/51248?article_type=v

Percutaneous Contrast Echocardiography-guided Intramyocardial Injection and Cell Delivery in a Large Preclinical Model

Read Article