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Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
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Chapters
- 00:05Title
- 01:25Purifying Schistosoma mansoni Eggs
- 04:00Intraperitoneal Sensitization with S. mansoni Eggs: Day 0
- 04:34Intravenous Challenge with S. mansoni Eggs: Day 14
- 05:06Experimental Harvest: Day 22
- 07:06Preparation of Recovered Tissues
- 07:53Results: Inflammatory Response to S. mansoni Infection
- 09:31Conclusion
Schistosoma mansoni eggs are potent stimulators of the T helper type 2 (Th2) immune response, characteristic of parasite infection, asthma and allergic inflammation. This protocol utilizes S. mansoni egg injection to generate a CD4 Th2 cytokine-induced inflammatory response in the lung, characterized by lung granuloma formation around the egg, eosinophilia and macrophage alternative activation.
Tags
Schistosoma MansoniHelminth-induced Lung InflammationParasite EggsImmune ResponseTh2 ResponseIL-4IL-5IL-13Alternative Activation Of MacrophagesEosinophilsPulmonary GranulomasAirway InflammationIntraperitoneal SensitizationIntravenous ChallengeLung ParenchymaLymphocytesCD4 T Cell ActivationIsolating Parasite EggsBroncho-alveolar Lavage (BAL)Analysis
