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Immunology and Infection

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JoVE Journal Immunology and Infection
Affinitetsrensning af Influenzavirus ribonukleoprotein komplekser fra kromatin af inficerede celler
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Affinitetsrensning af Influenzavirus ribonukleoprotein komplekser fra kromatin af inficerede celler

Article DOI: 10.3791/4028-v • 11:20 min • June 3rd, 2012
June 3rd, 2012 •
1, 1
1Department of Virology, Universitätsklinikum Freiburg

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Influenzavirus replikere deres RNA-genom i association med værts-celle kromatin. Her præsenterer vi en metode til at rense intakte virale ribonucleoprotein komplekser fra kromatin af inficerede celler. Oprenset virus komplekser kan analyseres ved både Western blot og primerforlængelse af protein og RNA-indhold, hhv.

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Virology Molecular Biology Influenza A virus affinitetsoprensning subcellulær fraktionering chromatin vRNP komplekser polymerase
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