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JoVE Journal Immunology and Infection
Affiniteit Zuivering van Influenza Virus ribonucleoproteïne Complexen van het chromatine van geïnfecteerde cellen
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Affiniteit Zuivering van Influenza Virus ribonucleoproteïne Complexen van het chromatine van geïnfecteerde cellen

Article DOI: 10.3791/4028-v • 11:20 min • June 3rd, 2012
June 3rd, 2012 •
1, 1
1Department of Virology, Universitätsklinikum Freiburg

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Influenza virussen repliceren hun RNA genoom in samenwerking met gastheer-cel chromatine. Hier we een methode intact viraal ribonucleoproteïne complexen zuiveren van chromatine van geïnfecteerde cellen. Gezuiverd virale complexen kunnen worden geanalyseerd door zowel Western blot en primer extension eiwit en RNA inhoud respectievelijk.

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Virologie Moleculaire Biologie Influenza A-virus affiniteitszuivering subcellulaire fractionering chromatine vRNP complexen polymerase
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