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Protein Transfection of Mouse Lung
JoVE Journal
Biology
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JoVE Journal Biology
Protein Transfection of Mouse Lung
DOI:

04:21 min

May 15, 2013

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Chapters

  • 00:05Title
  • 01:13Reagent Preparation
  • 02:29Intratracheal Administration of Protein
  • 03:36Results: Protein Transfection in the Lung
  • 03:58Conclusion

Summary

Automatic Translation

Transgenic mice or viral vectors have been used to increase protein expression within the lung. However, these techniques are time-consuming, technically challenging and have off-target effects that can confound results. Our protein transfection protocol uses a lipid based transfection reagent and an ultrafine microsprayer to uniformly deliver active protein to lung cells.

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