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Single Cell Transfection in Chick Embryos

Single Cell Transfection in Chick Embryos

Article DOI: 10.3791/2133
September 25th, 2010


Summary September 25th, 2010

Using fine tip micropipettes we inject plasmid DNA into subdomains of chicken somites or neural tubes. The concentration of the plasmid is adjusted to generate single transfected cells. We then allow the cells to develop into clonal populations.

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