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Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
JoVE Journal
Environment
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JoVE Journal Environment
Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
DOI:

15:23 min

October 01, 2013

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Chapters

  • 00:05Title
  • 01:41Conducting the Enzyme Assay for Soils and Sediments
  • 05:49Setting up Water Samples, Sample, Standard, Quench, and Substrate Controls
  • 08:54Recording Fluorescence
  • 09:50Calculation of Enzyme Activity per Volume of Water
  • 10:42Results: Extracellular Enzyme Activity in Waters, Soils, and Sediments
  • 14:44Conclusion

Summary

Automatic Translation

Microplate based procedures are described for the colorimetric or fluorometric analysis of extracellular enzyme activity. These procedures allow for the rapid assay of such activity in large numbers of environmental samples within a manageable time frame.

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