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The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells
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Biology
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The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

DOI:
10.3791/50610-v

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09:33 min

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September 20, 2013

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, , , ,
1Laboratory of Molecular Biology of the Cell,Ecole Normale Supérieure de Lyon, 2INSERM U744, Institut Pasteur de Lille,Université Lille-Nord de France, 3Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer,The Rockefeller University

Chapters

  • 00:05Title
  • 02:14Preparing Drosophila for Photoreceptor Visualization
  • 04:29Visualizing the Photoreceptors
  • 06:01Time-course Visualization of Photoreceptor Cells
  • 07:14Results from a High-throughput Screen
  • 09:13Conclusion

Summary

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The Tomato/GFP-FLP/FRT method involves visualizing mosaic photoreceptor cells in living Drosophila. It can be used to follow individual photoreceptor cell fates in the retina for days or weeks. This method is ideal for studies of retinal degeneration and neurodegenerative diseases or photoreceptor cell development.

Tags

DrosophilaPhotoreceptor CellsEyeClonal AnalysisLive ImagingTomato/GFP-FLP/FRTCornea NeutralizationMosaicNeuronal DegenerationPlanar Cell Polarity

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