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Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
DOI:

09:42 min

August 07, 2013

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Chapters

  • 00:05Title
  • 02:15Slice Preparation
  • 03:54Astrocyte Identification and Recordings
  • 04:46Isolation of the Facilitated Portion of Synaptically-activated Transporter Currents
  • 07:15Isolation of Flash-activated Transporter Currents
  • 07:58Results: Deconvolution Analysis
  • 08:58Conclusion

Summary

Automatic Translation

We describe an analytical method to estimate the lifetime of glutamate at astrocytic membranes from electrophysiological recordings of glutamate transporter currents in astrocytes.

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