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Super-resolution Imaging of Neuronal Dense-core Vesicles
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Super-resolution Imaging of Neuronal Dense-core Vesicles
DOI:

09:30 min

July 02, 2014

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Chapters

  • 00:05Title
  • 01:12Image Acquisition
  • 05:36Image Processing, Display, and Analysis
  • 07:07Results: Representative PALM Images
  • 09:04Conclusion

Summary

Automatic Translation

We describe how to implement photoactivated localization microscopy (PALM)-based studies of vesicles in fixed, cultured neurons. Key components of our protocol include labeling vesicles with photoconvertible chimeras, collecting sparsely sampled raw images with a super-resolution microscopy system, and processing the raw images to produce a super-resolution image.

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