Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Biochemical Assays for Analyzing Activities of…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights (Video) | JoVE
Journal (Biology)
Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights (Video) | JoVE
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
Journal (Biology)
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
 

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Article DOI: 10.3791/51721-v • 10:14 min • October 25th, 2014
October 25th, 2014 •
1,2, 1, 1,2, 1,2
1Stowers Institute for Medical Research, 2Department of Biochemistry & Molecular Biology, Kansas University Medical Center

Chapters

Summary
Automatic Translation

Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.

Tags

Biochemical Assays ATP-dependent Chromatin Remodeling Enzymes SNF2 Family Nucleosome Dynamics DNA Accessibility ATP-dependent Nucleosome Remodeling Multi-step ATP-dependent Chromatin Remodeling Reaction Reaction Products Reaction Intermediates Chromatin Remodeling Complexes Subcomplexes Subunits Nucleosome Sliding Assay Electrophoretic Mobility Shift Assay (EMSA) Nucleosome Binding Activity Thin Layer Chromatography (TLC) ATPase Activity DNA-dependent ATPase Activity Nucleosome-dependent ATPase Activity
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter