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Chapters
- 00:05Title
- 01:15ATP-dependent Nucleosome Remodeling Assays
- 02:55ATP-dependent Nucleosome Sliding Assays
- 05:11DNA- and Nucleosome-dependent ATPase Assays
- 07:15Defining Biochemical Activities of the INO80 Complex and INO80 Subcomplexes
- 09:17Conclusion
Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.
