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Zink-finger Nuclease Enhanced Gene Targeting i mänskliga embryonala stamcellslinjer
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Zink-finger Nuclease Enhanced Gene Targeting i mänskliga embryonala stamcellslinjer

Article DOI: 10.3791/51764-v • 12:13 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1, 1, 1
1Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University

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Reporter cellinjer erbjuder ett sätt att visualisera, spåra och isolera celler av intresse från heterogena populationer. Dock är gen-inriktning med konventionell homolog rekombination i humana embryonala stamceller extremt ineffektivt. Häri beskriver vi riktar CNS hjärnan specifik transkriptionsfaktor PITX3 locus med EGFP användning av zink-finger nukleas förstärkt homolog rekombination.

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Molecular Biology Elektroporation mänskliga embryonala stamceller genomet redigering reporter cellinje mitthjärnan dopaminerga neuroner
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