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Real-time Imaging af Plant Cell Surface Dynamics med variabel vinkel epifluorescens Microscopy
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Real-time Imaging af Plant Cell Surface Dynamics med variabel vinkel epifluorescens Microscopy

Article DOI: 10.3791/53437-v • 06:31 min • December 12th, 2015
December 12th, 2015 •
1
1Graduate School of Frontier Sciences, The University of Tokyo

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Målet med denne protokol er at demonstrere, hvordan at overvåge fluorescens-mærkede protein dynamik på plante celle overflader med variabel vinkel epifluorescens mikroskopi, der viser blinkende prikker af GFP-mærkede PATROL1, et protein-membran menneskehandel i cellen cortex i stomatal kompleks i Arabidopsis thaliana.

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Plantebiologi , Cell overflade exocytose Fluorescerende proteiner Billedanalyse Kymograph Membrane menneskehandel Plant cellebiologi Real-time imaging spalteåbninger Total indre refleksion mikroskopi variabel vinkel epifluorescens mikroskopi
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