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具有可变角度荧光显微镜植物细胞表面动力学的实时成像
JoVE Journal
Biology
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JoVE Journal Biology
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
DOI:

06:31 min

December 12, 2015

Chapters

  • 00:05Title
  • 00:35Cotyledon specimen Sky Drop Mounting
  • 02:35Variable-angle Epifluorescence Microscopy (VAEM) Observation and Movie Acquisition
  • 03:42GFP-labeled Dot Residence Time Quantification by Kymograph Analysis
  • 05:19Results: Representative GFP-PATROL1 Dot Residence Time Analysis by Kymography
  • 06:02Conclusion

Summary

Automatic Translation

该协议的目的是演示如何监测荧光标记蛋白动力学上的植物细胞表面与可变角荧光显微镜,表示闪烁GFP标记PATROL1,一个膜运输蛋白质的点,在所述气孔络合物在细胞皮质拟南芥。

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