Journal
/
Biology
/
Real-time Imaging af Plant Cell Surface Dynamics med variabel vinkel epifluorescens Microscopy
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

Real-time Imaging af Plant Cell Surface Dynamics med variabel vinkel epifluorescens Microscopy

DOI:
10.3791/53437-v

•

06:31 min

•

December 12, 2015

•

1Graduate School of Frontier Sciences,The University of Tokyo

Chapters

  • 00:05Title
  • 00:35Cotyledon specimen Sky Drop Mounting
  • 02:35Variable-angle Epifluorescence Microscopy (VAEM) Observation and Movie Acquisition
  • 03:42GFP-labeled Dot Residence Time Quantification by Kymograph Analysis
  • 05:19Results: Representative GFP-PATROL1 Dot Residence Time Analysis by Kymography
  • 06:02Conclusion

Summary

Automatic Translation

Automatic Translation

Målet med denne protokol er at demonstrere, hvordan at overvåge fluorescens-mærkede protein dynamik på plante celle overflader med variabel vinkel epifluorescens mikroskopi, der viser blinkende prikker af GFP-mærkede PATROL1, et protein-membran menneskehandel i cellen cortex i stomatal kompleks i Arabidopsis thaliana.

Tags

Cell Surface DynamicsVariable-angle Epifluorescence MicroscopyVAEMReal-time ImagingPlant Cell BiologyMembrane TraffickingCytoskeletal RearrangementFluorescently-labeled ProteinsPATROL1Stomatal MovementPlasma MembraneArabidopsis ThalianaKymograph AnalysisTime-lapse Imaging

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell

Aviditet baseret ekstracellulær interaktion Screening (AVEXIS) for skalerbar detektion af lav affinitet Ekstracellulære Receptor-Ligand Interactions

Tandem Højtryks-Frysning og Quick Freeze Substitution af plantevævene for Transmission Electron Microscopy

Realtidsmålinger membranprotein: Receptor Interactions Brug overfladeplasmonresonans (SPR)

Real Time Analyse af metaboliske profil i<em> Ex Vivo</em> Mus Intestinal Crypt organoide Cultures

Visualisere Stromule Frekvens med Fluorescence Microscopy

Light Sheet Fluorescens mikroskopi af planterødder Dyrkning på overfladen af ​​en gel

Flash-og-Freeze: en ny teknik til Capture Membrane Dynamics med Electron Microscopy

Konfokal mikroskopi afslører celle overflade Receptor sammenlægning gennem billedet korrelation spektroskopi

Read Article