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Real-time Imaging af Plant Cell Surface Dynamics med variabel vinkel epifluorescens Microscopy
JoVE Journal
Biology
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JoVE Journal Biology
Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy
DOI:

06:31 min

December 12, 2015

Chapters

  • 00:05Title
  • 00:35Cotyledon specimen Sky Drop Mounting
  • 02:35Variable-angle Epifluorescence Microscopy (VAEM) Observation and Movie Acquisition
  • 03:42GFP-labeled Dot Residence Time Quantification by Kymograph Analysis
  • 05:19Results: Representative GFP-PATROL1 Dot Residence Time Analysis by Kymography
  • 06:02Conclusion

Summary

Automatic Translation

Målet med denne protokol er at demonstrere, hvordan at overvåge fluorescens-mærkede protein dynamik på plante celle overflader med variabel vinkel epifluorescens mikroskopi, der viser blinkende prikker af GFP-mærkede PATROL1, et protein-membran menneskehandel i cellen cortex i stomatal kompleks i Arabidopsis thaliana.

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