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Journal / Genetics / As análises siRNA transfecção e EMSA sobre…
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JoVE Journal Genetics
As análises siRNA transfecção e EMSA sobre recentemente isolados das vilosidades citotrofoblastos Humanos
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As análises siRNA transfecção e EMSA sobre recentemente isolados das vilosidades citotrofoblastos Humanos

Article DOI: 10.3791/53995-v • 09:57 min • September 20th, 2016
September 20th, 2016 •
1, 2, 3, 1
1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

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Este protocolo descreve um método para eficazmente transfectar siARN em citotrofoblastos vilosidades humanos isolados de fresco utilizando formação de microporos e identificação de complexos de ADN-proteína nestas células. As células transfectadas pode ser monitorado por análises Western blot e EMSA durante o tempo de cultura de 4 dias.

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Genetics edição 115 a biologia molecular a emissão citotrofoblastos vilosidades Humanos Syncytin-2 siRNA microporos transfecção à base de lípidos eletroforética Mobilidade Mudança de Ensaio
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