De Voorbereiding van de Oblique Spinal Cord Slices voor Ventral Root Stimulation

1Centre National de la Recherche Scientifique (UMR 8119), Centre de Neurophysique, Physiologie et Pathologie, Université Paris Descartes
Neuroscience

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Leroy, F., Lamotte d'Incamps, B. The Preparation of Oblique Spinal Cord Slices for Ventral Root Stimulation. J. Vis. Exp. (116), e54525, doi:10.3791/54525 (2016).

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Abstract

Materials

Name Company Catalog Number Comments
Na-kynurenate ABCAM ab120256 dissolves better then other brands
KCl Sigma P3911
NaH2PO4 Sigma P5655
sucrose  Sigma S9378
NaHCO3  Sigma S6014
CaCl2  G Biosciences R040
MgCl2  Quality Biological 351-033-721
glucose  Sigma G5767
ascorbic acid  Sigma A5960
Na-pyruvate  Sigma P2250
K-gluconate  Sigma P1847
EGTA  Sigma E3889
HEPES  Sigma H4034
NaCl Sigma S9888
Agar Sigma A9799
QX-314 Alomone Q150
Mg-ATP Sigma A9187
CsOH Sigma 232041
Na-GTP Sigma 51120
gluconic acid Sigma G1951
Cesium hydroxide solution Sigma 232041
KOH Sigma P5958
Vannas Spring Scissors - 2.5mm  FST 15000-08 only use for cutting the dura, might get damaged if cutting bones
Stimulator A-M Systems Isolated Pulse Stimulator Model 2100
Vibratome Campden Vibrating Microtome 7000 - Model 7000smz-2

DOWNLOAD MATERIALS LIST

References

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